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AKT2 antibody [4H7]

Overexpression
Anti-AKT2 antibody [4H7] used in Flow cytometry (FACS). GTX84908
Anti-AKT2 antibody [4H7] used in Western Blot (WB). GTX84908
Anti-AKT2 antibody [4H7] used in Western Blot (WB). GTX84908
Anti-AKT2 antibody [4H7] used in IHC (Paraffin sections) (IHC-P). GTX84908
Anti-AKT2 antibody [4H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84908
Anti-AKT2 antibody [4H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84908
Anti-AKT2 antibody [4H7] used in Flow cytometry (FACS). GTX84908
Anti-AKT2 antibody [4H7] used in IHC (Paraffin sections) (IHC-P). GTX84908

Cat. No. GTX84908

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($519)
See all AKT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA

Cellular Localization

Cytoplasm,Nucleus

Background

General protein kinase capable of phosphorylating several known proteins.

Database

Research Area

DATA IMAGES

Anti-AKT2 antibody [4H7] used in Flow cytometry (FACS). GTX84908

GTX84908 FACS Image

FACS analysis of HeLa cells using GTX84908 AKT2 antibody [4H7].
Red : Primary antibody
Blue : Negative control antibody

Anti-AKT2 antibody [4H7] used in Western Blot (WB). GTX84908

GTX84908 WB Image

WB analysis of various cell lines using GTX84908 AKT2 antibody [4H7].
Loading : 10 ug per lane
Dilution : 1:200

Anti-AKT2 antibody [4H7] used in Western Blot (WB). GTX84908

GTX84908 WB Image

Overexpression

WB analysis of HEK293T cells transfected with AKT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84908 AKT2 antibody [4H7].
Loading : 5 ug per lane

Anti-AKT2 antibody [4H7] used in IHC (Paraffin sections) (IHC-P). GTX84908

GTX84908 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84908 AKT2 antibody [4H7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-AKT2 antibody [4H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84908

GTX84908 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84908 AKT2 antibody [4H7].

Anti-AKT2 antibody [4H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84908

GTX84908 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with AKT2 plasmid using GTX84908 AKT2 antibody [4H7].

Anti-AKT2 antibody [4H7] used in Flow cytometry (FACS). GTX84908

GTX84908 FACS Image

FACS analysis of Jurkat cells using GTX84908 AKT2 antibody [4H7].
Red : Primary antibody
Blue : Negative control antibody

Anti-AKT2 antibody [4H7] used in IHC (Paraffin sections) (IHC-P). GTX84908

GTX84908 IHC-P Image

IHC-P analysis of human liver tissue using GTX84908 AKT2 antibody [4H7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519