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AKT2 antibody

KO/KD Overexpression
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Anti-AKT2 antibody used in Western Blot (WB). GTX128457

Cat. No. GTX128457

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all AKT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Raw264.7 , C2C12 , PC-12 , HA-human AKT2-transfected 293T cells

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Akt2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2750992

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:500

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg Raw264.7 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:500

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Overexpression

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells
C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells
7.5 % SDS-PAGE
Akt2 antibody (GTX128457) dilution: 1:20000

Anti-AKT2 antibody used in Western Blot (WB). GTX128457

GTX128457 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zemanovic S et al. Cell Rep 2018; 25 (9) : 2605-2616.e7 Dynamic Phosphorylation of the C Terminus of Hsp70 Regulates the Mitochondrial Import of SOD2 and Redox Balance.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

AKT2 antibody Cat. No. GTX128457

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-AKT2 antibody used in Western Blot (WB). GTX128457
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169