APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HA-human AKT2-transfected 293T cells
Product Note
Knockdown/Knockout validation was supported by customer review data.
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Akt2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885782
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA
Background
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX128458 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT2 antibody (GTX128458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128458 WB Image
Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells
C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells
7.5 % SDS-PAGE
Akt2 antibody (GTX128458) dilution: 1:20000
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GTX128458 IHC-P Image
AKT2 antibody detects AKT2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
AKT2 antibody (GTX128458) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for AKT2 antibody (GTX128458). Be the first to share your publications with this product.
REVIEW
AKT2 antibody
Cat. No. GTX128458
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 27-Oct-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
HEK293 cells
Amount used :
50μg
Blocking :
5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
