GeneTex

  • Country / Location Selection

United States (US)

AKT2 antibody

KO/KD Overexpression
Anti-AKT2 antibody used in Western Blot (WB). GTX128458
Anti-AKT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128458
Anti-AKT2 antibody used in Western Blot (WB). GTX128458

Cat No. GTX128458

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all AKT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Akt2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKT2 antibody used in Western Blot (WB). GTX128458

GTX128458 WB Image

Overexpression

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HA-human Akt1-transfected 293T cells
C. 5 μg whole cell lysate/extract of HA-human Akt2-transfected 293T cells
7.5 % SDS-PAGE
Akt2 antibody (GTX128458) dilution: 1:20000

Anti-AKT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128458

GTX128458 IHC-P Image

AKT2 antibody detects AKT2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
AKT2 antibody (GTX128458) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AKT2 antibody used in Western Blot (WB). GTX128458

GTX128458 WB Image

Akt2 antibody detects Akt2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
7.5 % SDS-PAGE
Akt2 antibody (GTX128458) dilution: 1:1000

REFERENCE

There are currently no references for AKT2 antibody (GTX128458). Be the first to share your publications with this product.
Submit a Reference

REVIEW

AKT2 antibody Cat No. GTX128458

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-AKT2 antibody used in Western Blot (WB). GTX128458
Date : Anonymous submitted on 27-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169