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AKT3 antibody [9B2]

Overexpression
Anti-AKT3 antibody [9B2] used in Western Blot (WB). GTX84906
Anti-AKT3 antibody [9B2] used in IHC (Paraffin sections) (IHC-P). GTX84906
Anti-AKT3 antibody [9B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84906
Anti-AKT3 antibody [9B2] used in Western Blot (WB). GTX84906
Anti-AKT3 antibody [9B2] used in Flow cytometry (FACS). GTX84906
Anti-AKT3 antibody [9B2] used in Western Blot (WB). GTX84906

Cat. No. GTX84906

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9B2

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($519)
See all AKT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 3 , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2

Cellular Localization

Nucleus,Cytoplasm,Membrane

Background

IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Database

Research Area

DATA IMAGES

Anti-AKT3 antibody [9B2] used in Western Blot (WB). GTX84906

GTX84906 WB Image

Overexpression

WB analysis of HEK293T cells transfected with AKT3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84906 AKT3 antibody [9B2].
Loading : 5 ug per lane

Anti-AKT3 antibody [9B2] used in IHC (Paraffin sections) (IHC-P). GTX84906

GTX84906 IHC-P Image

IHC-P analysis of human lung tissue using GTX84906 AKT3 antibody [9B2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-AKT3 antibody [9B2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84906

GTX84906 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with AKT3 plasmid using GTX84906 AKT3 antibody [9B2].

Anti-AKT3 antibody [9B2] used in Western Blot (WB). GTX84906

GTX84906 WB Image

WB analysis of SVT2 and PC12 cell lysate using GTX84906 AKT3 antibody [9B2].
Loading : 10 ug per lane
Dilution : 1:200

Anti-AKT3 antibody [9B2] used in Flow cytometry (FACS). GTX84906

GTX84906 FACS Image

FACS analysis of HEK293T cells transfected with either AKT3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84906 AKT3 antibody [9B2].

Anti-AKT3 antibody [9B2] used in Western Blot (WB). GTX84906

GTX84906 WB Image

WB analysis of BALB/C 3T3 cell lysate using GTX84906 AKT3 antibody [9B2].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519