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ALAS-E antibody [N3C3]

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139
Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139
Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

Cat No. GTX104139

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Raji , K562 , HL60 , mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALAS-E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

5'-aminolevulinate synthase 2 , ALAS-E , ALASE , ANH1 , ASB , SIDBA1 , XLDPP , XLEPP , XLSA

Cellular Localization

Mitochondrion matrix

Background

The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

GTX104139 WB Image

The WB analysis of ALAS-E antibody [N3C3] was published by Vercellotti GM and colleagues in the journal Front Pharmacol in 2014.PMID: 24860503

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

GTX104139 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX104139 diluted at 1:1000

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

GTX104139 WB Image

Sample (30 ug of whole cell lysate)
A: K562
7.5% SDS PAGE
GTX104139 diluted at 1:500

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf