APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml for 30 min at RT |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 315-434) of human ALDH1A1 protein (exact sequence is proprietary).
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ALDC , ALDH1 , ALDH11 , ALDH1A1 , ALDHE1 , HEL12 , HEL9 , HELS53e , PUMB1 , RALDH1 , aldehyde dehydrogenase 1 family member A1
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX34400 WB Image
WB analysis of K562 and lung lysates using GTX34400 ALDH1A1 antibody [ALDH1A1/1381].
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GTX34400 IHC-P Image
IHC-P analysis of human testicular carcinoma tissue using GTX34400 ALDH1A1 antibody [ALDH1A1/1381].
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GTX34400 WB Image
WB analysis of human liver tissue lysate using GTX34400 ALDH1A1 antibody [ALDH1A1/1381].
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GTX34400 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34400 ALDH1A1 antibody [ALDH1A1/1381].
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REFERENCE
There are currently no references for ALDH1A1 antibody [ALDH1A1/1381] (GTX34400). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ALDH1A1 antibody [ALDH1A1/1381] (GTX34400). Be the first to share your experience with this product.