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ALDH1A1 antibody, C-term

Anti-ALDH1A1 antibody, C-term used in Western Blot (WB). GTX90658

Cat. No. GTX90658

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX90658-PEP).

Product Note

This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EVKTVTVKISQKNS, from the C Terminus of the protein sequence according to NP_000680.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1

Cellular Localization

Cytoplasm, cytosol

Background

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

Database

  • Gene ID: 216 ALDH1A1
  • UniProt: P00352 ALDH1A1

Research Area

DATA IMAGES

Anti-ALDH1A1 antibody, C-term used in Western Blot (WB). GTX90658

GTX90658 WB Image

WB analysis of human (A), mouse (B) and rat (C) liver lysate using GTX90658 ALDH1A1 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399