GeneTex
United States (US)

ALDH1A1 antibody [C3], C-term

GTX100043_IHC_19011506_490.jpg
GTX100043_39233_IFA_19011506_702.jpg
GTX100043_39233_WB_M_liver_19011506_237.jpg
GTX100043_39233_IHC_M_19011506_558.jpg
GTX100043_39233_WB_19011506_320.jpg

Cat No. GTX100043

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rabbit, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ALDH1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALDC antibody, ALDH-E1 antibody, ALDH1 antibody, ALDH11 antibody, MGC2318 antibody, PUMB1 antibody, RALDH1 antibody, ALDH1A1 antibody, ALHDII antibody, ALDH class 1 antibody, retinaldehyde dehydrogenase 1 antibody, acetaldehyde dehydrogenase 1 antibody, aldehyde dehydrogenase 1, soluble antibody, RALDH 1 antibody, aldehyde dehydrogenase, liver cytosolic antibody, retinal dehydrogenase 1 antibody, aldehyde dehydrogenase 1 family, member A1 antibody

Cellular Localization

Cytoplasm

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100043_IHC_19011506_490.jpg

GTX100043 IHC-P Image

Immunohistochemical analysis of paraffin-embedded gastric N87 xenograft, using ALDH1A1(GTX100043) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100043_39233_IFA_19011506_702.jpg

GTX100043 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ALDH1A1(GTX100043) antibody at 1:200 dilution.

GTX100043_39233_WB_M_liver_19011506_237.jpg

GTX100043 WB Image

ALDH1A1 antibody [C3], C-term detects ALDH1A1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
ALDH1A1 antibody [C3], C-term (GTX100043) dilution: 1:10000

GTX100043_39233_IHC_M_19011506_558.jpg

GTX100043 IHC-P Image

ALDH1A1 antibody [C3], C-term detects ALDH1A1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ALDH1A1 antibody [C3], C-term (GTX100043) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100043_39233_WB_19011506_320.jpg

GTX100043 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100043 diluted at 1:10000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169