APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89404-PEP).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RELGEYGFHEYTE, from the internal region of the protein sequence according to NP_000680.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1
Cellular Localization
Cytoplasm, cytosol
Background
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX89404 IHC-P Image
IHC-P analysis of human testis using GTX89404 ALDH1A1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX89404 WB Image
WB analysis of human liver lysate using GTX89404 ALDH1A1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89404 WB Image
WB analysis of HEK293 overexpressing ALDH1A1 (mock transfection in first lane) using GTX89404 ALDH1A1 antibody, Internal.
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REFERENCE
There are currently no references for ALDH1A1 antibody, Internal (GTX89404). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ALDH1A1 antibody, Internal (GTX89404). Be the first to share your experience with this product.
