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ALDH1A1 antibody

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973
Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123973
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

Cat No. GTX123973

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549 , mouse liver , rat liver , *Huh7 , *HepG2

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aldehyde dehydrogenase 1 family member A1,ALDC,ALDH-E1,ALDH1,ALDH11,HEL-9,HEL-S-53e,HEL12,PUMB1,RALDH1

Cellular Localization

Cytoplasm

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Database

  • Gene ID: 216 ALDH1A1
  • UniProt: P00352 ALDH1A1

Research Area

DATA IMAGES
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.
A. 50 µg rat liver lysate/extract
10% SDS-PAGE
ALDH1A1 antibody (GTX123973) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123973

GTX123973 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ALDH1A1(GTX123973) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis.
A. 50 µg mouse liver lysate/extract
10% SDS-PAGE
ALDH1A1 antibody (GTX123973) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123973

GTX123973 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1(GTX123973) antibody at 1:500 dilution.

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123973

GTX123973 WB Image

Sample (30 µg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX123973 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

ALDH1A1 antibody Cat No. GTX123973

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : T24 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 24-Dec-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells, HepG2 cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane A: HepG2 cells; Lane B: Huh7 cells
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169