GeneTex
United States (US)

ALDH1A1 antibody

GTX123974_40737_IFA_18121410_167.jpg
GTX123974_40737_IHC_18121410_235.jpg
GTX123974_40737_WB_18121410_693.jpg

Cat No. GTX123974

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALDC antibody, ALDH-E1 antibody, ALDH1 antibody, ALDH11 antibody, MGC2318 antibody, PUMB1 antibody, RALDH1 antibody, ALDH1A1 antibody, ALHDII antibody, ALDH class 1 antibody, retinaldehyde dehydrogenase 1 antibody, acetaldehyde dehydrogenase 1 antibody, "aldehyde dehydrogenase 1, soluble antibody", RALDH 1 antibody, "aldehyde dehydrogenase, liver cytosolic antibody", retinal dehydrogenase 1 antibody, "aldehyde dehydrogenase 1 family, member A1 antibody"

Cellular Localization

Cytoplasm

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX123974_40737_IFA_18121410_167.jpg

GTX123974 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1(GTX123974) antibody at 1:500 dilution.

GTX123974_40737_IHC_18121410_235.jpg

GTX123974 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ALDH1A1(GTX123974) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123974_40737_WB_18121410_693.jpg

GTX123974 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX123974 diluted at 1:5000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169