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ALDH1A1 antibody

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123974
Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123974
Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123974
Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123974

Cat. No. GTX123974

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all ALDH1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1

Cellular Localization

Cytoplasm

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]

Database

  • Gene ID: 216 ALDH1A1
  • UniProt: P00352 ALDH1A1

Research Area

DATA IMAGES

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123974

GTX123974 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123974) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123974

GTX123974 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ALDH1A1(GTX123974) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH1A1 antibody used in Western Blot (WB). GTX123974

GTX123974 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX123974 diluted at 1:5000

Anti-ALDH1A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123974

GTX123974 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1(GTX123974) antibody at 1:500 dilution.

REFERENCE

Application Reference

Ma L et al. Lung Cancer Manag 2019; 8 (1) : LMT04 Targeting claudin-3 suppresses stem cell-like phenotype in nonsquamous non-small-cell lung carcinoma.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169