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ALDH1A3 antibody [GT926]

Anti-ALDH1A3 antibody [GT926] used in Western Blot (WB). GTX633822
Anti-ALDH1A3 antibody [GT926] used in IHC (Paraffin sections) (IHC-P). GTX633822
Anti-ALDH1A3 antibody [GT926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633822
Anti-ALDH1A3 antibody [GT926] used in Western Blot (WB). GTX633822
Anti-ALDH1A3 antibody [GT926] used in IHC (Paraffin sections) (IHC-P). GTX633822
Anti-ALDH1A3 antibody [GT926] used in Western Blot (WB). GTX633822

Cat. No. GTX633822

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT926

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ALDH1A3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Huh-7 , U87-MG , SK-N-AS , A431

Predict Reactivity

Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888385

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A3 , ALDH1A6 , ALDH6 , MCOP8 , RALDH3

Cellular Localization

Cytoplasm

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Database

  • Gene ID: 220 ALDH1A3
  • UniProt: P47895 ALDH1A3

Research Area

DATA IMAGES

Anti-ALDH1A3 antibody [GT926] used in Western Blot (WB). GTX633822

GTX633822 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ALDH1A3 antibody [GT926] used in IHC (Paraffin sections) (IHC-P). GTX633822

GTX633822 IHC-P Image

ALDH1A3 antibody [GT926] detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
ALDH1A3 stained by ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1A3 antibody [GT926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633822

GTX633822 ICC/IF Image

ALDH1A3 antibody [GT926] detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALDH1A3 protein stained by ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ALDH1A3 antibody [GT926] used in Western Blot (WB). GTX633822

GTX633822 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ALDH1A3 antibody [GT926] used in IHC (Paraffin sections) (IHC-P). GTX633822

GTX633822 IHC-P Image

ALDH1A3 antibody [GT926] detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH1A3 stained by ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1A3 antibody [GT926] used in Western Blot (WB). GTX633822

GTX633822 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [GT926] (GTX633822) diluted at 1:3000.

REFERENCE

Application Reference

Park JW et al. Oncotarget 2017; 8 (59) : 99382-99393 Inhibition of aldehyde dehydrogenase 1 enhances the cytotoxic effect of retinaldehyde on A549 cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations