GeneTex
United States (US)

ALDH1A3 antibody [N2C2], Internal

GTX110784_42992_20171006_WB_19011507_749.jpg
GTX110784_40394_IHC_19011507_496.jpg
GTX110784_41605_20170209_WB_19011507_274.jpg
GTX110784_42992_20180112_WB_19011507_661.jpg
GTX110784_43082_20180314_ICC_IF_19011507_646.jpg

Cat No. GTX110784

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
See all ALDH1A3 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

A431 , Huh-7 , U87-MG

Predict Reactivity

Mouse, Rat, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALDH1A6 antibody, ALDH6 antibody, RALDH3 antibody, ALDH1A3 antibody, RALDH-3 antibody, aldehyde dehydrogenase 6 antibody, retinaldehyde dehydrogenase 3 antibody, aldehyde dehydrogenase family 1 member A3 antibody, acetaldehyde dehydrogenase 6 antibody, "aldehyde dehydrogenase 1 family, member A3 antibody"

Cellular Localization

Cytoplasm

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX110784_42992_20171006_WB_19011507_749.jpg

GTX110784 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110784_40394_IHC_19011507_496.jpg

GTX110784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH1A3(GTX110784) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX110784_41605_20170209_WB_19011507_274.jpg

GTX110784 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000.
GTX110784_42992_20180112_WB_19011507_661.jpg

GTX110784 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX110784_43082_20180314_ICC_IF_19011507_646.jpg

GTX110784 ICC/IF Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.
REFERENCE

Application Reference

Chen MH et al. Clin Cancer Res 2016; (Epub ahead of print) ALDH1A3, the major aldehyde dehydrogenase isoform in human cholangiocarcinoma cells, affects prognosis and gemcitabine resistance in cholangiocarcinoma patients.
Application : IHC-P
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169