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ALDH1A3 antibody [N2C2], Internal

Orthogonal
Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784

Cat. No. GTX110784

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ALDH1A3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Huh-7 , U87-MG

Predict Reactivity

Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727704

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A3 , ALDH1A6 , ALDH6 , MCOP8 , RALDH3

Cellular Localization

Cytoplasm

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Database

  • Gene ID: 220 ALDH1A3
  • UniProt: P47895 ALDH1A3

Research Area

DATA IMAGES

Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784

GTX110784 IHC-P Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

GTX110784 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ALDH1A3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110784

GTX110784 ICC/IF Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

GTX110784 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

GTX110784 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000.

Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784

GTX110784 IHC-P Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Shin-Yi Chung et al. Biomedicines 2021; 9 (8)
Application : IHC-P
Reactivity : Human
Park JW et al. Sci Rep 2019; 9 (1) : 6462 ATP-binding Cassette Transporters Substantially Reduce Estimates of ALDH-positive Cancer Cells based on Aldefluor and AldeRed588 Assays.
Application : WB
Reactivity : Human
Chen MH et al. Clin Cancer Res 2016; (Epub) aldh1a3, the major aldehyde dehydrogenase isoform in human cholangiocarcinoma cells, affects prognosis and * resistance in cholangiocarcinoma patients.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169