ALDH1A3 antibody [N2C2], Internal

Cat No. GTX110784

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat

See all ALDH1A3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

A431 , Huh-7 , U87-MG

Predict Reactivity

Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Aldehyde Dehydrogenase 1 Family Member A3,Aldh1A6,Aldh6,Mcop8,Raldh3,Aldh1A3

Cellular Localization

Cytoplasm

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Database

Gene ID: 220
UniProt: P47895

Research Area

DATA IMAGES

GTX110784 IHC-P Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110784 IHC-P Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110784 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110784 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000.

GTX110784 WB Image

GTX110784 ICC/IF Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.