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ALDH1A3 antibody [N2C2], Internal

Orthogonal
Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784
Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

Cat No. GTX110784

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all ALDH1A3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Huh-7 , U87-MG

Predict Reactivity

Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727704

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member A3 , ALDH1A6 , ALDH6 , MCOP8 , RALDH3

Cellular Localization

Cytoplasm

Background

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Database

  • Gene ID: 220 ALDH1A3
  • UniProt: P47895 ALDH1A3

Research Area

DATA IMAGES

Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

GTX110784 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

GTX110784 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:2000.

Anti-ALDH1A3 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110784

GTX110784 ICC/IF Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784

GTX110784 IHC-P Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1A3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110784

GTX110784 IHC-P Image

ALDH1A3 antibody [N2C2], Internal detects ALDH1A3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH1A3 stained by ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1A3 antibody [N2C2], Internal used in Western Blot (WB). GTX110784

GTX110784 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], Internal (GTX110784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shin-Yi Chung et al. Biomedicines 2021; 9 (8) Ruxolitinib Combined with Gemcitabine against Cholangiocarcinoma Growth via the JAK2/STAT1/3/ALDH1A3 Pathway.
Application : IHC-P
Reactivity : Human
Park JW et al. Sci Rep 2019; 9 (1) : 6462 ATP-binding Cassette Transporters Substantially Reduce Estimates of ALDH-positive Cancer Cells based on Aldefluor and AldeRed588 Assays.
Application : WB
Reactivity : Human
Chen MH et al. Clin Cancer Res 2016; (Epub) aldh1a3, the major aldehyde dehydrogenase isoform in human cholangiocarcinoma cells, affects prognosis and * resistance in cholangiocarcinoma patients.
Application : IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169