GeneTex
United States (US)

ALDH1B1 antibody

GTX107279_39995_20150518_IHC_M_19011506_591.jpg
GTX107279_39995_IFA_19011506_688.jpg
GTX107279_39995_IHC_19011506_635.jpg
GTX107279_WB_19011506_791.jpg

Cat No. GTX107279

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldehyde Dehydrogenase 1 Family Member B1,Aldh5,Aldhx,Aldh1B1

Cellular Localization

Mitochondrion matrix

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107279_39995_20150518_IHC_M_19011506_591.jpg

GTX107279 IHC-P Image

ALDH1B1 antibody detects ALDH1B1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ALDH1B1 antibody (GTX107279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107279_39995_IFA_19011506_688.jpg

GTX107279 ICC/IF Image

ALDH1B1 antibody detects ALDH1B1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ALDH1B1 protein stained by ALDH1B1 antibody (GTX107279) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107279_39995_IHC_19011506_635.jpg

GTX107279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ALDH1B1(GTX107279) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107279_WB_19011506_791.jpg

GTX107279 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX107279 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169