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ALDH1B1 antibody

Anti-ALDH1B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107279
Anti-ALDH1B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107279
Anti-ALDH1B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107279
Anti-ALDH1B1 antibody used in Western Blot (WB). GTX107279

Cat. No. GTX107279

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ALDH1B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949627

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member B1 , ALDH5 , ALDHX

Cellular Localization

Mitochondrion matrix

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]

Database

  • Gene ID: 219 ALDH1B1
  • UniProt: P30837 ALDH1B1

Research Area

DATA IMAGES

Anti-ALDH1B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107279

GTX107279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ALDH1B1(GTX107279) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107279

GTX107279 ICC/IF Image

ALDH1B1 antibody detects ALDH1B1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ALDH1B1 protein stained by ALDH1B1 antibody (GTX107279) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ALDH1B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107279

GTX107279 IHC-P Image

ALDH1B1 antibody detects ALDH1B1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ALDH1B1 antibody (GTX107279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ALDH1B1 antibody used in Western Blot (WB). GTX107279

GTX107279 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1B1 antibody (GTX107279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Eman MS Muhammad et al. SOHAG MEDICAL JOURNAL 2019; 22 : No.3 Immunohistochemical Expression of Cancer Stem Cell marker Aldehyde dehydrogenase 1B1 (ALDH1B1) in Colorectal Carcinoma.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169