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ALDH1L1 antibody

Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047
Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047
Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047
Anti-ALDH1L1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131047
Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047

Cat No. GTX131047

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

mouse liver , mouse brain , rat brain , rat kidney

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886410

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aldehyde dehydrogenase 1 family member L1 , 10-FTHFDH , 10-fTHF , FDH , FTHFD

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047

GTX131047 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1L1 antibody (GTX131047) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047

GTX131047 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1L1 antibody (GTX131047) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047

GTX131047 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1L1 antibody (GTX131047) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH1L1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX131047

GTX131047 IHC-Fr Image

ALDH1L1 antibody detects ALDH1L1 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: ALDH1L1 protein stained by ALDH1L1 antibody (GTX131047) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ALDH1L1 antibody used in Western Blot (WB). GTX131047

GTX131047 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1L1 antibody (GTX131047) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169