APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/75 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aldehyde dehydrogenase 2 family member , ALDH-E2 , ALDHI , ALDM
Cellular Localization
Mitochondrion matrix
Background
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
![Anti-ALDH2 antibody [4G6A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60727](/templates/default/js/lightbox/images/loading.gif "Anti-ALDH2 antibody [4G6A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60727")
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GTX60727 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX60727 ALDH2 antibody [4G6A3].
Green : ALDH2
Blue: DRAQ5 fluorescent DNA dye
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GTX60727 FACS Image
FACS analysis of HeLa cells using GTX60727 ALDH2 antibody [4G6A3].
Green : ALDH2
Red : negative control
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GTX60727 WB Image
WB analysis of HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate using GTX60727 ALDH2 antibody [4G6A3].
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GTX60727 WB Image
WB analysis of HepG2 (1), A549 (2) cell lysate, and Rat liver (3), Mouse liver (4), Mouse brain (5) tissue lysate using GTX60727 ALDH2 antibody [4G6A3].
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GTX60727 ELISA Image
ELISA analysis of antigen using GTX60727 ALDH2 antibody [4G6A3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60727 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60727 ALDH2 antibody [4G6A3].
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GTX60727 IHC-P Image
IHC-P analysis of cervical cancer tissue using GTX60727 ALDH2 antibody [4G6A3].
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REFERENCE
There are currently no references for ALDH2 antibody [4G6A3] (GTX60727). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ALDH2 antibody [4G6A3] (GTX60727). Be the first to share your experience with this product.
![Anti-ALDH2 antibody [4G6A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_ICCIF_w_23061123_475.webp "Anti-ALDH2 antibody [4G6A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Flow cytometry (FACS). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_FACS_w_23061123_611.webp "Anti-ALDH2 antibody [4G6A3] used in Flow cytometry (FACS). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_WB_w_23061123_793.webp "Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_WB_1_w_23061123_567.webp "Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in ELISA (ELISA). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_ELISA_w_23061123_129.webp "Anti-ALDH2 antibody [4G6A3] used in ELISA (ELISA). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_IHC-P_1_w_23061123_678.webp "Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727](/upload/website/prouct_img/small/GTX60727/GTX60727_20170912_IHC-P_w_23061123_348.webp "Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_ICCIF_w_23061123_475.webp "Anti-ALDH2 antibody [4G6A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Flow cytometry (FACS). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_FACS_w_23061123_611.webp "Anti-ALDH2 antibody [4G6A3] used in Flow cytometry (FACS). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_WB_w_23061123_793.webp "Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_WB_1_w_23061123_567.webp "Anti-ALDH2 antibody [4G6A3] used in Western Blot (WB). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in ELISA (ELISA). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_ELISA_w_23061123_129.webp "Anti-ALDH2 antibody [4G6A3] used in ELISA (ELISA). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_IHC-P_1_w_23061123_678.webp "Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727")
![Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727](/upload/website/prouct_img/normal/GTX60727/GTX60727_20170912_IHC-P_w_23061123_348.webp "Anti-ALDH2 antibody [4G6A3] used in IHC (Paraffin sections) (IHC-P). GTX60727")