GeneTex
United States (US)

ALDH2 antibody, Internal

GTX88073_2541_WB_18121411_383.jpg
GTX88073_20_ELISA_18121411_971.jpg

Cat No. GTX88073

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, sELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture : GTX101429 , Detection : GTX88073

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88073-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_000681.2; NP_001191818.1).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DETQFKKILGYIN, from the internal region of the protein sequence according to NP_000681.2; NP_001191818.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldehyde Dehydrogenase 2 Family Member,Aldh-E2,Aldhi,Aldm,Aldh2

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES
GTX88073_2541_WB_18121411_383.jpg

GTX88073 WB Image

WB analysis of mouse liver lysate using GTX88073 ALDH2 antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

GTX88073_20_ELISA_18121411_971.jpg

GTX88073 ELISA Image

ELISA analysis of ALDH2 protein using GTX88073 ALDH2 antibody, Internal.
Capture : GTX101429 (2.5μg/ml)
Detection : GTX88073 (1.5μg/ml)

Package List Price ($)
$ 329