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ALDH2 antibody [N1C1]

Anti-ALDH2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113379
Anti-ALDH2 antibody [N1C1] used in Western Blot (WB). GTX113379
Anti-ALDH2 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX113379
Anti-ALDH2 antibody [N1C1] used in Western Blot (WB). GTX113379
Anti-ALDH2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113379
Anti-ALDH2 antibody [N1C1] used in Western Blot (WB). GTX113379

Cat. No. GTX113379

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ALDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Mouse brain , HepG2

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036201

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 2 family member , ALDH-E2 , ALDHI , ALDM

Cellular Localization

Mitochondrion matrix

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALDH2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113379

GTX113379 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody [N1C1] (GTX113379) at 1:300 dilution.

Anti-ALDH2 antibody [N1C1] used in Western Blot (WB). GTX113379

GTX113379 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
GTX113379 diluted at 1:1000

Anti-ALDH2 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX113379

GTX113379 IHC-Wm Image

ALDH2 antibody [N1C1] detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH2 antibody [N1C1] (GTX113379) dilution: 1:200.
Anti-ALDH2 antibody [N1C1] used in Western Blot (WB). GTX113379

GTX113379 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX113379 diluted at 1:1000

Anti-ALDH2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113379

GTX113379 ICC/IF Image

ALDH2 antibody [N1C1] detects ALDH2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: ALDH2 protein stained by ALDH2 antibody [N1C1] (GTX113379) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-ALDH2 antibody [N1C1] used in Western Blot (WB). GTX113379

GTX113379 WB Image

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113379 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169