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ALDH2 antibody

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in ELISA (ELISA). GTX101429

Cat No. GTX101429

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, sELISA
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX101429 , Detection: GTX88073

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

GL261 , C8D30 , BCL-1 , Raw264.7 , HepG2 , HepG2 cytoplasm extract

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldehyde Dehydrogenase 2 Family Member,Aldh-E2,Aldhi,Aldm,Aldh2

Cellular Localization

Mitochondrion matrix

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2(GTX101429) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

ALDH2 antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH2 antibody (GTX101429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101429

GTX101429 ICC/IF Image

ALDH2 antibody detects ALDH2 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALDH2 protein stained by ALDH2 antibody (GTX101429) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

HepG2 whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

ALDH2 antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ALDH2 antibody (GTX101429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysae/extract
D. 30 µg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
ALDH2 antibody (GTX101429) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH2 antibody used in ELISA (ELISA). GTX101429

GTX101429 ELISA Image

ELISA detection of ALDH2 using GTX101429 for capture at a concentration of 2.5 µg/mL and GTX88073 for detection at a concentration of 1.5 µg/mL.

REFERENCE
REVIEW

ALDH2 antibody Cat No. GTX101429

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : MIN6 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169