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ALDH3A1 antibody [1B6]

Overexpression
Anti-ALDH3A1 antibody [1B6] used in Western Blot (WB). GTX84889
Anti-ALDH3A1 antibody [1B6] used in Western Blot (WB). GTX84889
Anti-ALDH3A1 antibody [1B6] used in Flow cytometry (FACS). GTX84889
Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889
Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889
Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889
Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889
Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889
Anti-ALDH3A1 antibody [1B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84889

Cat. No. GTX84889

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B6

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all ALDH3A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ALDH3A1(NP_000682) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 3 family member A1 , ALDH3 , ALDHIII

Cellular Localization

Cytoplasm

Background

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

Database

  • Gene ID: 218 ALDH3A1
  • UniProt: P30838 ALDH3A1

Research Area

DATA IMAGES

Anti-ALDH3A1 antibody [1B6] used in Western Blot (WB). GTX84889

GTX84889 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ALDH3A1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84889 ALDH3A1 antibody [1B6].
Loading : 5 ug per lane

Anti-ALDH3A1 antibody [1B6] used in Western Blot (WB). GTX84889

GTX84889 WB Image

WB analysis of HeLa and A549 cell lysate using GTX84889 ALDH3A1 antibody [1B6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-ALDH3A1 antibody [1B6] used in Flow cytometry (FACS). GTX84889

GTX84889 FACS Image

FACS analysis of HEK293T cells transfected with either ALDH3A1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84889 ALDH3A1 antibody [1B6].

Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889

GTX84889 IHC-P Image

IHC-P analysis of prostate tissue using GTX84889 ALDH3A1 antibody [1B6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889

GTX84889 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84889 ALDH3A1 antibody [1B6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889

GTX84889 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84889 ALDH3A1 antibody [1B6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889

GTX84889 IHC-P Image

IHC-P analysis of liver tissue using GTX84889 ALDH3A1 antibody [1B6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ALDH3A1 antibody [1B6] used in IHC (Paraffin sections) (IHC-P). GTX84889

GTX84889 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84889 ALDH3A1 antibody [1B6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ALDH3A1 antibody [1B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84889

GTX84889 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ALDH3A1 plasmid using GTX84889 ALDH3A1 antibody [1B6].

REFERENCE

Application Reference

Sharifi S et al. ACS Appl Bio Mater 2022; 5 (12) : 5716-5727 Electrospun-Reinforced Suturable Biodegradable Artificial Cornea.
Mohammad Mirazul Islam et al. Sci Rep 2022; 12 (1) : 9108 Crosslinker-free collagen gelation for corneal regeneration.
Application : ICC/IF
Reactivity : Human
Sina Sharifi et al. Macromol Biosci 2021; 21 (4) : e2000379 Electron Beam Sterilization of Poly(Methyl Methacrylate)-Physicochemical and Biological Aspects.
Application : ICC/IF
Reactivity : Human
Sina Sharifi et al. Bioact Mater 2021; 6 (11) : 3947-3961 Tuning gelatin-based hydrogel towards bioadhesive ocular tissue engineering applications.
Application : IHC-P
Reactivity : Human
Sina Sharifi et al. ACS Appl Bio Mater 2021; 4 (10) : 7682-7691 Photo-cross-linked Gelatin Glycidyl Methacrylate/N-Vinylpyrrolidone Copolymeric Hydrogel with Tunable Mechanical Properties for Ocular Tissue Engineering Applications.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519