Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody [N1C2] (GTX112391) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of frozen bovine cornea section, using ALDH3A1(GTX112391) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody (GTX112391) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.