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ALDH3A1 antibody [N1C2]

Anti-ALDH3A1 antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX112391
Anti-ALDH3A1 antibody [N1C2] used in Western Blot (WB). GTX112391
Anti-ALDH3A1 antibody [N1C2] used in Western Blot (WB). GTX112391

Cat. No. GTX112391

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Mouse, Bovine, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Human, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH3A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617821

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 3 family member A1 , ALDH3 , ALDHIII

Cellular Localization

Cytoplasm

Background

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

  • Gene ID: 218 ALDH3A1
  • UniProt: P30838 ALDH3A1

Research Area

DATA IMAGES

Anti-ALDH3A1 antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX112391

GTX112391 IHC-Fr Image

Immunohistochemical analysis of frozen bovine cornea section, using ALDH3A1(GTX112391) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH3A1 antibody [N1C2] used in Western Blot (WB). GTX112391

GTX112391 WB Image

Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody [N1C2] (GTX112391) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH3A1 antibody [N1C2] used in Western Blot (WB). GTX112391

GTX112391 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody (GTX112391) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

ALDH3A1 antibody [N1C2] Cat. No. GTX112391

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Oct-2011
Score :
Application Tested : IHC-Fr
Sample Species : Bov
Sample : Whole Cornea tissue
Blocking : 5% bovine serum abumin in PBS, 25°C, 2Hr
Fixation : 3.7% PFA in PBS
Permeabilization /Antigen retrieval : 0.1% Trition X
Primary Antibody : 1:100, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169