ALDH5A1 antibody [N1C1]

Name: ALDH5A1 antibody [N1C1]
Brand: GeneTex
SKU: GTX110181
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ALDH5A1 antibody [N1C1] used in Western Blot (WB). GTX110181

Anti-ALDH5A1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110181

Anti-ALDH5A1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX110181

Cat. No. GTX110181

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX110181 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Mouse brain , A431

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH5A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949630

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 5 family member A1 , SSADH , SSDH

Cellular Localization

Mitochondrion

Background

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Gene ID: 7915 ALDH5A1
UniProt: P51649 ALDH5A1

Research Area

DATA IMAGES

GTX110181 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX110181 diluted at 1:1000

GTX110181 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ALDH5A1(GTX110181) antibody at 1:500 dilution.

GTX110181 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ALDH5A1(GTX110181) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110181 WB Image

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110181 diluted at 1:5000

GTX110181 IHC-P Image

ALDH5A1 antibody [N1C1] detects ALDH5A1 protein at mitochondria in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ALDH5A1 antibody [N1C1] (GTX110181) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Martirosian V et al. Cell Rep 2021; 35 (13) : 109302 Medulloblastoma uses GABA transaminase to survive in the cerebrospinal fluid microenvironment and promote leptomeningeal dissemination.

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REVIEW