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ALDH7A1 antibody [C1C3]

Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274
Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274
Anti-ALDH7A1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114274
Anti-ALDH7A1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114274
Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274
Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274
Anti-ALDH7A1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114274

Cat. No. GTX114274

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ALDH7A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Mouse liver , HeLa , rat liver

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH7A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619649

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldehyde dehydrogenase 7 family member A1 , ATQ1 , EPD , PDE

Background

Antiquitin is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. Mutations in this gene cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride. Four additional human antiquitin-like sequences, all of which are pseudogenes, have also been identified. [provided by RefSeq]

Database

  • Gene ID: 501 ALDH7A1
  • UniProt: P49419 ALDH7A1

Research Area

DATA IMAGES

Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274

GTX114274 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH7A1 antibody [C1C3] (GTX114274) diluted at 1:3000.

Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274

GTX114274 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX114274 diluted at 1:10000

Anti-ALDH7A1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114274

GTX114274 IHC-P Image

ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ALDH7A1 antibody [C1C3] (GTX114274) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH7A1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114274

GTX114274 ICC/IF Image

ALDH7A1 antibody [C1C3] detects ALDH7A1 protein at mitochondria and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: ALDH7A1 protein stained by ALDH7A1 antibody [C1C3] (GTX114274) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274

GTX114274 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
ALDH7A1 antibody
GTX114274 diluted at 1:1000

Anti-ALDH7A1 antibody [C1C3] used in Western Blot (WB). GTX114274

GTX114274 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX114274 diluted at 1:1000

Anti-ALDH7A1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114274

GTX114274 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH7A1 antibody [C1C3] (GTX114274) at 1:300 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169