GeneTex
United States (US)

ALDH7A1 antibody

GTX103593_40688_IHC_19011506_766.jpg
GTX103593_40688_IFA_19011506_842.jpg
GTX103593_40688_20150821_IHC-P_M_19011506_848.jpg
GTX103593_40688_WB_19011506_319.jpg

Cat No. GTX103593

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH7A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldehyde Dehydrogenase 7 Family Member A1,Atq1,Epd,Pde,Aldh7A1

Background

Antiquitin is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. Mutations in this gene cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride. Four additional human antiquitin-like sequences, all of which are pseudogenes, have also been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103593_40688_IHC_19011506_766.jpg

GTX103593 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using ALDH7A1(GTX103593) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103593_40688_IFA_19011506_842.jpg

GTX103593 ICC/IF Image

ALDH7A1 antibody detects ALDH7A1 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: ALDH7A1 protein stained by ALDH7A1 antibody (GTX103593) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103593_40688_20150821_IHC-P_M_19011506_848.jpg

GTX103593 IHC-P Image

ALDH7A1 antibody detects ALDH7A1 protein at cytoplasm in mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ALDH7A1 antibody (GTX103593) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103593_40688_WB_19011506_319.jpg

GTX103593 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX103593 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169