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ALIX antibody [3A9]

Orthogonal
Anti-ALIX antibody [3A9] used in Western Blot (WB). GTX42812
Anti-ALIX antibody [3A9] used in Western Blot (WB). GTX42812

Cat. No. GTX42812

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Xenopus laevis
Package
100 μg ($399)
See all ALIX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Product Note

This antibody recognizes the apoptosis-linked gene-2 interacting protein X (ALIX). Clone 3A9 has been used extensively for the detection of ALIX by Western blotting and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Alix fusion protein.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11162693

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 6 interacting protein , AIP1 , ALIX , DRIP4 , HP95

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-ALIX antibody [3A9] used in Western Blot (WB). GTX42812

GTX42812 WB Image

WB analysis of HeLa cell lysate using GTX42812 ALIX antibody [3A9].

Anti-ALIX antibody [3A9] used in Western Blot (WB). GTX42812

GTX42812 WB Image

Orthogonal

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 31324203

REFERENCE

Application Reference

HK Jackson et al. J Extracell Vesicles 2024; 13 (2) : e12412 Bioengineered small extracellular vesicles deliver multiple SARS-CoV-2 antigenic fragments and drive a broad immunological response
Application : WB
Zhang Q et al. J Exp Clin Cancer Res 2019; 38 (1) : 320 Exosomal transfer of p-STAT3 promotes acquired 5-FU resistance in colorectal cancer cells.
Application : WB
Reactivity : Human
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399