Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ALIX antibody (GTX135282) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ALIX antibody detects ALIX protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
ALIX stained by ALIX antibody (GTX135282) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min