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ALIX antibody

Anti-ALIX antibody used in IHC (Paraffin sections) (IHC-P). GTX64925
Anti-ALIX antibody used in IHC (Paraffin sections) (IHC-P). GTX64925
Anti-ALIX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64925
Anti-ALIX antibody used in IHC (Paraffin sections) (IHC-P). GTX64925
Anti-ALIX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64925
Anti-ALIX antibody used in Western Blot (WB). GTX64925

Cat. No. GTX64925

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ALIX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PDCD6IP (NP_037506.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 6 interacting protein , AIP1 , ALIX , DRIP4 , HP95

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-ALIX antibody used in IHC (Paraffin sections) (IHC-P). GTX64925

GTX64925 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64925 ALIX antibody.
Dilution : 1:100

Anti-ALIX antibody used in IHC (Paraffin sections) (IHC-P). GTX64925

GTX64925 IHC-P Image

IHC-P analysis of human stomach tissue using GTX64925 ALIX antibody.
Dilution : 1:100

Anti-ALIX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64925

GTX64925 ICC/IF Image

ICC/IF analysis of U-251 MG cells using GTX64925 ALIX antibody.
Blue : DAPI
Dilution : 1:100

Anti-ALIX antibody used in IHC (Paraffin sections) (IHC-P). GTX64925

GTX64925 IHC-P Image

IHC-P analysis of human prostate tissue using GTX64925 ALIX antibody.
Dilution : 1:100

Anti-ALIX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64925

GTX64925 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64925 ALIX antibody.
Blue : DAPI
Dilution : 1:100

Anti-ALIX antibody used in Western Blot (WB). GTX64925

GTX64925 WB Image

WB analysis of various sample lysates using GTX64925 ALIX antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399