GeneTex
United States (US)

ALK (phospho Tyr1604) antibody

Orthogonal
GTX16377_20190308_WB_19030804_863.jpg
GTX16377_20190308_ICCIF_19030804_441.jpg
GTX16377_20190308_WB_1_19030804_665.jpg
GTX16377_20190308_IHC-P_19030804_806.jpg

Cat No. GTX16377

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:100
IHC-Fr Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 1604 (G-H-Y(p)-E-D) derived from human ALK.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALK,CD246,NBLST3,anaplastic lymphoma receptor tyrosine kinase

Cellular Localization

Cell membrane

Background

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES
GTX16377_20190308_WB_19030804_863.jpg

GTX16377 WB Image

Orthogonal

WB analysis of extracts from COS-7 cells (lane 2) using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

GTX16377_20190308_ICCIF_19030804_441.jpg

GTX16377 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

GTX16377_20190308_WB_1_19030804_665.jpg

GTX16377 WB Image

WB analysis of extracts from HepG2 cells using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

GTX16377_20190308_IHC-P_19030804_806.jpg

GTX16377 IHC-P Image

IHC-P analysis of human brain tissue using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE
Package List Price ($)
$ 319