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ALKBH2 antibody

Anti-ALKBH2 antibody used in Western Blot (WB). GTX85166
Anti-ALKBH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85166

Cat. No. GTX85166

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ALKBH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995) , Human Kidney Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ALKBH2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ALKBH2.The immunogen is located within amino acids 180 - 230 of ALKBH2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkB homolog 2, alpha-ketoglutarate dependent dioxygenase , ABH2

Cellular Localization

Nucleus

Background

The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.

Database

Research Area

DATA IMAGES

Anti-ALKBH2 antibody used in Western Blot (WB). GTX85166

GTX85166 WB Image

WB analysis of human kidney tissue lysate using GTX85166 ALKBH2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-ALKBH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85166

GTX85166 IHC-P Image

IHC-P analysis of human kidney tissue using GTX85166 ALKBH2 antibody.
Working concentration : 10 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399