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ALOX12 antibody, C-term

Anti-ALOX12 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80966
Anti-ALOX12 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX80966
Anti-ALOX12 antibody, C-term used in Flow cytometry (FACS). GTX80966
Anti-ALOX12 antibody, C-term used in Western Blot (WB). GTX80966

Cat. No. GTX80966

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all ALOX12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11174765

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arachidonate 12-lipoxygenase, 12S type , 12-LOX , 12S-LOX , LOG12

Cellular Localization

Cytoplasm, cytosol,Membrane

Background

This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients. Allelic variants in this gene may be associated with susceptibility to toxoplasmosis. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ALOX12 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80966

GTX80966 ICC/IF Image

ICC/IF analysis of A549 cells using GTX80966 ALOX12 antibody, C-term.
Green : ALOX12
Red : Actin filament
Dilution : 1:100

Anti-ALOX12 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX80966

GTX80966 IHC-P Image

IHC-P analysis of human skin tissue using GTX80966 ALOX12 antibody, C-term.

Anti-ALOX12 antibody, C-term used in Flow cytometry (FACS). GTX80966

GTX80966 FACS Image

FACS analysis of K562 cells using GTX80966 ALOX12 antibody, C-term.
Top histogram : negative control
Bottom histogram : K562 cells

Anti-ALOX12 antibody, C-term used in Western Blot (WB). GTX80966

GTX80966 WB Image

WB analysis of K562 cell lysate (35ug/lane) using GTX80966 ALOX12 antibody, C-term.

REFERENCE

Application Reference

Zhong C et al. Oncol Lett 2018; 16 (2) : 1455-1462 12-Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial-mesenchymal transition.
Zhong C et al. Oncology Letters 2018; (Epub) 12-Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial-mesenchymal transition.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399