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ALOX15 antibody, Internal

Anti-ALOX15 antibody, Internal used in Western Blot (WB). GTX88501
Anti-ALOX15 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88501
Anti-ALOX15 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88501

Cat. No. GTX88501

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ALOX15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-0.5μg/ml
IHC-P 2μg/ml
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88501-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HYKTDVAVKDDPE, from the internal region of the protein sequence according to NP_001131.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arachidonate 15-lipoxygenase , 12-LOX , 15-LOX , 15-LOX-1 , LOG15

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ALOX15 antibody, Internal used in Western Blot (WB). GTX88501

GTX88501 WB Image

WB analysis of HeLa nuclear lysate using GTX88501 ALOX15 antibody, Internal.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer

Anti-ALOX15 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88501

GTX88501 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX88501 ALOX15 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-ALOX15 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88501

GTX88501 IHC-P Image

IHC-P analysis of human kidney using GTX88501 ALOX15 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399