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ALP / PDLIM3 antibody [C2C3], C-term

Anti-ALP / PDLIM3 antibody [C2C3], C-term used in Western Blot (WB). GTX107697
Anti-ALP / PDLIM3 antibody [C2C3], C-term used in Western Blot (WB). GTX107697

Cat. No. GTX107697

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all ALP / PDLIM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

PDLIM3 transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ALP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951163

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PDZ and LIM domain 3 , ALP

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALP / PDLIM3 antibody [C2C3], C-term used in Western Blot (WB). GTX107697

GTX107697 WB Image

Western Blot analysis of ALP / PDLIM3 expression in transfected 293T cell line by ALP / PDLIM3 antibody [C2C3], C-term (GTX107697).
A: Non-transfected lysate.
B: ALP / PDLIM3 transfected lysate. 12% SDS PAGE
GTX107697 diluted at 1:500

Anti-ALP / PDLIM3 antibody [C2C3], C-term used in Western Blot (WB). GTX107697

GTX107697 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ALP / PDLIM3 antibody [C2C3], C-term (GTX107697) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169