GeneTex

  • Country / Location Selection

United States (US)

ALPPL2 antibody

Orthogonal Overexpression
Anti-ALPPL2 antibody used in Western Blot (WB). GTX114250
Anti-ALPPL2 antibody used in Western Blot (WB). GTX114250
Anti-ALPPL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114250
Anti-ALPPL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114250
Anti-ALPPL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114250

Cat. No. GTX114250

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, EM

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ALPPL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
EM Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALPPL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, germ cell , ALPPL , ALPPL2 , GCAP

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALPPL2 antibody used in Western Blot (WB). GTX114250

GTX114250 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALPPL2 antibody (GTX114250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALPPL2 antibody used in Western Blot (WB). GTX114250

GTX114250 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALPPL2 antibody (GTX114250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ALPPL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114250

GTX114250 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using ALPPL2(GTX114250) antibody at 1:500 dilution.

Anti-ALPPL2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114250

GTX114250 ICC/IF Image

ALPPL2 antibody detects ALPPL2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALPPL2 protein stained by ALPPL2 antibody (GTX114250) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-ALPPL2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114250

GTX114250 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Breast, using ALPPL2(GTX114250) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Odorfer KI et al. J Cell Mol Med 2011; 15 (10) : 2232-44 Hematopoietic bone marrow cells participate in endothelial, but not epithelial or mesenchymal cell renewal in adult rats.
Application : EM
Submit a Reference

REVIEW

There are currently no reviews for ALPPL2 antibody (GTX114250). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169