Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALPPL2 antibody (GTX114250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALPPL2 antibody (GTX114250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human Breast, using ALPPL2(GTX114250) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ALPPL2 antibody detects ALPPL2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ALPPL2 protein stained by ALPPL2 antibody (GTX114250) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Immunofluorescence analysis of methanol-fixed A549, using ALPPL2(GTX114250) antibody at 1:500 dilution.