APPLICATION
Application Note
Western blot: approx 35 kDa band seen in human heart and human liver extracts [Predicted MW of approx. 31 kDa for beta-isoform according to AAF71042]. Recommended for use at 0.2-1.0μg/ml. Please note that we have not yet observed the longer alpha-isoform of ILPIP in our experiments.
Calculated MW
Positive Control
Human heart or liver lysate.
Product Note
This is expected to recognise both human isoforms: ILPIP alpha (NP_061041) and ILPIP beta (AAF71042).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CDFPDEKDSYWEF, from C Terminus of the protein sequence according to AAF71042
Purification
IgG fraction
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
STE20 related adaptor beta , ALS2CR2 , CALS-21 , ILPIP , ILPIPA , PAPK , PRO1038
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Database
Research Area
DATA IMAGES
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GTX24122 WB Image
GTX24122 at a concentration of 1mg/ml staining ALS2CR2 inhuman heartlysate (RIPA buffer, 30mg total protein per lane) by Western blot.Primary incubated for1 hour and detecte
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GTX24122 IHC-P Image
IHC staining of ALS2CR2/ILPIP in paraffin embedded human heart using GTX24122 at 3.8μg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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REFERENCE
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REVIEW
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