GeneTex
United States (US)

AMACR antibody

GTX34409_20170712_IHC-P_1_18121410_669.jpg
GTX34409_20170712_IHC-P_18121410_575.jpg

Cat No. GTX34409

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:1:50-1:100 for 30-60 minutes at RT.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and Ī �-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide from human AMACR protein

Purification

Ab purified from rabbit anti-serum by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alpha-Methylacyl-Coa Racemase,Amacrd,Cbas4,P504S,Race,Rm,Amacr

Background

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES
GTX34409_20170712_IHC-P_1_18121410_669.jpg

GTX34409 IHC-P Image

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with AMACR / p504S Rabbit Polyclonal Antibody.

GTX34409_20170712_IHC-P_18121410_575.jpg

GTX34409 IHC-P Image

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR / p504S Rabbit Polyclonal Antibody.

Package List Price ($)
$ 329