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AMBRA1 antibody

Anti-AMBRA1 antibody used in Western Blot (WB). GTX129487

Cat No. GTX129487

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

human AMBRA1-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human AMBRA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy and beclin 1 regulator 1 , DCAF3 , WDR94

Cellular Localization

Cytoplasmic vesicle , autophagosome

Database

Research Area

DATA IMAGES

Anti-AMBRA1 antibody used in Western Blot (WB). GTX129487

GTX129487 WB Image

AMBRA1 antibody detects AMBRA1 protein by western blot analysis. Non-transfected (-) and AMBRA1-transfected (+, ) 293T whole cell extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AMBRA1 antibody (GTX129487) at a dilution of 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169