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AMBRA1 antibody

Anti-AMBRA1 antibody used in Western Blot (WB). GTX129487

Cat. No. GTX129487

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AMBRA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

human AMBRA1-transfected 293T cells

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human AMBRA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886013

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy and beclin 1 regulator 1 , DCAF3 , WDR94

Cellular Localization

Cytoplasmic vesicle , autophagosome

Database

Research Area

DATA IMAGES

Anti-AMBRA1 antibody used in Western Blot (WB). GTX129487

GTX129487 WB Image

AMBRA1 antibody detects AMBRA1 protein by western blot analysis. Non-transfected (-) and AMBRA1-transfected (+, ) 293T whole cell extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with AMBRA1 antibody (GTX129487) at a dilution of 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169