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AMBRA1 antibody

Anti-AMBRA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17003
Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003
Anti-AMBRA1 antibody used in Western Blot (WB). GTX17003
Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003

Cat. No. GTX17003

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all AMBRA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , 3T3 (NIH) Cell Lysate(GTX27179)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1.The immunogen is located within the last 50 amino acids of Ambra1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887550

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy and beclin 1 regulator 1 , DCAF3 , WDR94

Database

Research Area

DATA IMAGES

Anti-AMBRA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17003

GTX17003 ICC/IF Image

ICC/IF analysis of K562 cells using GTX17003 AMBRA1 antibody.
Dilution : 20 μg/ml

Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003

GTX17003 IHC-P Image

IHC-P analysis of human brain tissue using GTX17003 AMBRA1 antibody.
Working concentration : 5 μg/ml

Anti-AMBRA1 antibody used in Western Blot (WB). GTX17003

GTX17003 WB Image

WB analysis of 3T3 cell lysate using GTX17003 AMBRA1 antibody.
Working concentration : 1 μg/ml

Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003

GTX17003 IHC-P Image

IHC-P analysis of human brain tissue using GTX17003 AMBRA1 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Fauzia E et al. Front Pharmacol 2018; 9 : 1034 Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399