GeneTex
United States (US)

AMCR antibody

Cat No. GTX15894

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC, IM
Reactivity Human, Mouse
APPLICATION

Application Note

IHC: Use at a dilution of 1/100. IM: Use at an assay dependent dilution. WB: Use at a dilution of 1/500. Predicted molecular weight: 42 kDa corresponding to variant AMCR I. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

GTX15894 is specific for AMCR I
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: TDACVTPVLT FEEWHHD(C), corresponding to C Terminal amino acids 290-307 of Human AMCR, the peptide was modified post-synthesis.

Purification

Immunogen affinity purified
Affinity purified against immobilized antigen. Supplied as a pure IgG fraction.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alpha-Methylacyl-Coa Racemase,Amacrd,Cbas4,P504S,Race,Rm,Amacr

Cellular Localization

Mitochondrial

Background

Alpha-methyacyl-CoA racemase (AMCR), is an enzyme invloved in beta oxidation of branched chain fatty acids and bile salt intermediates, and has recently been identified as a neomarker for prostate cancer, where it is over expressed. Several different isoforms have been reported that are produced either by extensinve alternatve splicing of 5 exons or by use of alternate initiation codons. At least 2 different transcripts, each derived from the 5 exons, have been reported, AMCR I and AMCR II. AMCR I is the most abundant form and enclodes for a 382 amino acid protein (42kDa). The other isoform AMCR II exhibits significnat homolgy to fumerate hydratase and encoes a 288 amino acid protein (32 kDa). Several other variants of IA and IIA isoforms are charcterized recently. The variants lack exon 3 and are designated as IB and IIB. In prostate tumor tissues that over express AMCR, both the A and B forms are over-expressed.

Database

Research Area

Package List Price ($)
$ 359