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AMFR antibody [HL2865]

Recombinant antibody Orthogonal
Anti-AMFR antibody [HL2865] used in Western Blot (WB). GTX640142
Anti-AMFR antibody [HL2865] used in Western Blot (WB). GTX640142
Anti-AMFR antibody [HL2865] used in Western Blot (WB). GTX640142

Cat. No. GTX640142

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2865

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all AMFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of Human AMFR.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autocrine motility factor receptor , GP78 , RNF45

Cellular Localization

Endoplasmic reticulum membrane

Background

This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-AMFR antibody [HL2865] used in Western Blot (WB). GTX640142

GTX640142 WB Image

Orthogonal

Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2865] (GTX640142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMFR antibody [HL2865] used in Western Blot (WB). GTX640142

GTX640142 WB Image

Orthogonal

Boiled and unboiled rat tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2865] (GTX640142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMFR antibody [HL2865] used in Western Blot (WB). GTX640142

GTX640142 WB Image

Orthogonal

Boiled and unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2865] (GTX640142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Available soon.
Custom Conjugations