Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AMFR antibody [HL2866] detects AMFR protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: AMFR stained by AMFR antibody [HL2866] (GTX640143) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.