AMFR antibody [N3C3]

Name: AMFR antibody [N3C3]
Brand: GeneTex
SKU: GTX112393
Price: 379.00 USD
Rating: 5 (1 reviews)

Anti-AMFR antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112393

Anti-AMFR antibody [N3C3] used in Western Blot (WB). GTX112393

Cat No. GTX112393

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($379), 25 μl ($169)

See all AMFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Mouse, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172297

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autocrine motility factor receptor , GP78 , RNF45

Cellular Localization

Endoplasmic reticulum membrane

Background

Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. [provided by RefSeq]

Database

Gene ID: 267 AMFR
UniProt: Q9UKV5 AMFR

Research Area

DATA IMAGES

GTX112393 IHC-P Image

AMFR antibody [N3C3] detects AMFR protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
AMFR antibody [N3C3] (GTX112393) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112393 WB Image

Sample (30 ug of whole cell lysate)
A: K562
7.5% SDS PAGE
GTX112393 diluted at 1:1000

GTX112393 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using AMFR(GTX112393) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chang YW et al. Oncogene 2015; Deacetylation of HSPA5 by HDAC6 leads to GP78-mediated HSPA5 ubiquitination at K447 and suppresses metastasis of breast cancer.

Application : WB

Reactivity : Human

Chang YW et al. Oncotarget 2014; 5 (21) : 10558-70 De-acetylation and degradation of HSPA5 is critical for E1A metastasis suppression in breast cancer cells.

Application : WB

Reactivity : Human

Gelsomino G et al. Mol Cancer 2013; 12 : 137 Omega 3 fatty acids chemosensitize multidrug resistant colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition.

Application : WB

Reactivity : Human

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REVIEW