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AMFR antibody [N3C3]

Anti-AMFR antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112393
Anti-AMFR antibody [N3C3] used in Western Blot (WB). GTX112393
Anti-AMFR antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112393

Cat No. GTX112393

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Mouse, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMFR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172297

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autocrine motility factor receptor , GP78 , RNF45

Cellular Localization

Endoplasmic reticulum membrane

Background

Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMFR antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112393

GTX112393 IHC-P Image

AMFR antibody [N3C3] detects AMFR protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
AMFR antibody [N3C3] (GTX112393) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AMFR antibody [N3C3] used in Western Blot (WB). GTX112393

GTX112393 WB Image

Sample (30 ug of whole cell lysate)
A: K562
7.5% SDS PAGE
GTX112393 diluted at 1:1000

Anti-AMFR antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112393

GTX112393 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using AMFR(GTX112393) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

AMFR antibody [N3C3] Cat No. GTX112393

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2013. PMID: 24225025
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169