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AMFR antibody

Anti-AMFR antibody used in IHC (Paraffin sections) (IHC-P). GTX04315
Anti-AMFR antibody used in Flow cytometry (FACS). GTX04315
Anti-AMFR antibody used in IHC (Paraffin sections) (IHC-P). GTX04315
Anti-AMFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04315
Anti-AMFR antibody used in Flow cytometry (FACS). GTX04315
Anti-AMFR antibody used in IHC (Paraffin sections) (IHC-P). GTX04315
Anti-AMFR antibody used in Western Blot (WB). GTX04315

Cat. No. GTX04315

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all AMFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 5 μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells

Note :

IHC-P
Antigen retireval by heat
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived human AMFR recombinant protein (Position: E553-S643)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autocrine motility factor receptor , GP78 , RNF45

Cellular Localization

Endoplasmic reticulum membrane

Background

This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-AMFR antibody used in IHC (Paraffin sections) (IHC-P). GTX04315

GTX04315 IHC-P Image

IHC-P analysis of human placenta tissue using GTX04315 AMFR antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0)
Dilution : 1 μg/ml

Anti-AMFR antibody used in Flow cytometry (FACS). GTX04315

GTX04315 FACS Image

FACS analysis of SiHa cells using GTX04315 AMFR antibody.
Dilution : 1μg/1x106 cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-AMFR antibody used in IHC (Paraffin sections) (IHC-P). GTX04315

GTX04315 IHC-P Image

IHC-P analysis of mouse intestine tissue using GTX04315 AMFR antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6.0) for 20 mins
Dilution : 1 μg/ml

Anti-AMFR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04315

GTX04315 ICC/IF Image

ICC/IF analysis of T47D cells using GTX04315 AMFR antibody.
Green : Primary antibopdy
Blue : DAPI
Antigen retrieval : IHC enzyme antigen retrieval reagent for 15 mins
Dilution : 5 μg/mL

Anti-AMFR antibody used in Flow cytometry (FACS). GTX04315

GTX04315 FACS Image

FACS analysis of U87 cells using GTX04315 AMFR antibody.
Dilution : 1μg/1x106 cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-AMFR antibody used in IHC (Paraffin sections) (IHC-P). GTX04315

GTX04315 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04315 AMFR antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6.0) for 20 mins
Dilution : 1 μg/ml

Anti-AMFR antibody used in Western Blot (WB). GTX04315

GTX04315 WB Image

WB analysis of various cell lysates using GTX04315 AMFR antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Daudi whole cell lysate
Lane 4 : rat kidney tissue lysate
Dilution : 0.5 μg/mL
Loading : 30 μg

REFERENCE

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Package List Price ($)
$ 469