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AMH antibody

Anti-AMH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129593
Anti-AMH antibody used in Western Blot (WB). GTX129593
Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593
Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593

Cat No. GTX129593

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AMH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

anti-Mullerian hormone , MIF , MIS

Background

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129593

GTX129593 ICC/IF Image

AMH antibody detects AMH protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: AMH protein stained by AMH antibody (GTX129593) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-AMH antibody used in Western Blot (WB). GTX129593

GTX129593 WB Image

AMH antibody detects AMH protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
AMH antibody (GTX129593) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593

GTX129593 IHC-P Image

AMH antibody detects AMH protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
AMH antibody (GTX129593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593

GTX129593 IHC-P Image

AMH antibody detects AMH protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
AMH antibody (GTX129593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169