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AMH antibody

Anti-AMH antibody used in Western Blot (WB). GTX129593
Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593
Anti-AMH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129593
Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593

Cat. No. GTX129593

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Cat
Package
100 μl ($399),
25 μl ($169)
See all AMH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AMH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886044

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anti-Mullerian hormone , MIF , MIS

Background

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMH antibody used in Western Blot (WB). GTX129593

GTX129593 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AMH antibody (GTX129593) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593

GTX129593 IHC-P Image

AMH antibody detects AMH protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
AMH antibody (GTX129593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AMH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129593

GTX129593 ICC/IF Image

AMH antibody detects AMH protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: AMH protein stained by AMH antibody (GTX129593) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-AMH antibody used in IHC (Paraffin sections) (IHC-P). GTX129593

GTX129593 IHC-P Image

AMH antibody detects AMH protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
AMH antibody (GTX129593) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Mirzaeian L et al. J Ovarian Res 2023; 16 (1) : 56 In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix.
Application : IHC-P
Reactivity : Mouse
Nilg?n G?ltiken et al. Animals (Basel) 2022; 12 (7) Expression of Anti-M?llerian Hormone and Its Type 2 Receptor in the Ovary of Pregnant and Cyclic Domestic Cats.
Application : WB, IHC-P
Reactivity : Cat
Habara O et al. Development 2021; 148 (9) WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.
Application : IHC-P
Reactivity : Mouse
Hashemian Z et al. Cytotechnology 2020; Establishment and characterization of a PCOS and a normal human granulosa cell line.
Application : ICC/IF
Reactivity : Human
Atrabi MJ et al. J Cell Physiol 2018; (Epub) Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169