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AMID antibody [N1], N-term

Orthogonal
Anti-AMID antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108089
Anti-AMID antibody [N1], N-term used in Western Blot (WB). GTX108089

Cat No. GTX108089

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all AMID products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HepG2 , Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human AMID. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949610

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

apoptosis inducing factor mitochondria associated 2 , AMID , PRG3

Cellular Localization

Cytoplasm , Mitochondrion outer membrane

Background

The protein encoded by this gene has significant homology to NADH oxidoreductases and the apoptosis-inducing factor PDCD8/AIF. Overexpression of this gene has been shown to induce apoptosis. The expression of this gene is found to be induced by tumor suppressor protein p53 in colon caner cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMID antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108089

GTX108089 IHC-P Image

AMID antibody [N1], N-term detects AMID protein at cell membrane and cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded human gastric cancer.</br>AMID stained by AMID antibody [N1], N-term (GTX108089) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AMID antibody [N1], N-term used in Western Blot (WB). GTX108089

GTX108089 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with AMID antibody [N1], N-term (GTX108089) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 40 kDa, and 43 kDa. It is possibly due to alternative splicing.
Reference: PMID: 33254395

REFERENCE

Application Reference

Kanemura S et al. Cell Physiol Biochem 2015; 36 (3) : 1037-48 Phosphatidylinositol Induces Caspase-Independent Apoptosis of Malignant Pleural Mesothelioma Cells by Accumulating AIF in the Nucleus.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169