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AMPK alpha 1 + AMPK alpha 2 antibody

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50863
Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Western Blot (WB). GTX50863
Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50863

Cat No. GTX50863

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from human AMPK alpha 1/2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , protein kinase AMP-activated catalytic subunit alpha 2 , AMPK , AMPKa1 , AMPK , AMPK2 , AMPKa2 , PRKAA

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50863

GTX50863 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50863 AMPK alpha 1/2 antibody.

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in Western Blot (WB). GTX50863

GTX50863 WB Image

WB analysis of extracts from A549, HepG2, and 3T3 cells using GTX50863 AMPK alpha 1/2 antibody.

Anti-AMPK alpha 1 + AMPK alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50863

GTX50863 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50863 AMPK alpha 1/2 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf