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AMPK alpha 1 antibody [2B7]

Anti-AMPK alpha 1 antibody [2B7] used in IHC (Paraffin sections) (IHC-P). GTX60403
Anti-AMPK alpha 1 antibody [2B7] used in Flow cytometry (FACS). GTX60403
Anti-AMPK alpha 1 antibody [2B7] used in ELISA (ELISA). GTX60403
Anti-AMPK alpha 1 antibody [2B7] used in Western Blot (WB). GTX60403
Anti-AMPK alpha 1 antibody [2B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60403

Cat. No. GTX60403

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($409)
See all AMPK alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PRKAA1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887963

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 antibody [2B7] used in IHC (Paraffin sections) (IHC-P). GTX60403

GTX60403 IHC-P Image

IHC-P analysis of ovarian cancer (left) and brain tissue (right) using GTX60403 AMPK alpha 1 antibody [2B7].

Anti-AMPK alpha 1 antibody [2B7] used in Flow cytometry (FACS). GTX60403

GTX60403 FACS Image

FACS analysis of PC-2 cells using GTX60403 AMPK alpha 1 antibody [2B7].
Green : AMPK alpha 1
Purple : negative control

Anti-AMPK alpha 1 antibody [2B7] used in ELISA (ELISA). GTX60403

GTX60403 ELISA Image

ELISA analysis of antigen using GTX60403 AMPK alpha 1 antibody [2B7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-AMPK alpha 1 antibody [2B7] used in Western Blot (WB). GTX60403

GTX60403 WB Image

WB analysis of Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH-3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate using GTX60403 AMPK alpha 1 antibody [2B7].

Anti-AMPK alpha 1 antibody [2B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60403

GTX60403 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX60403 AMPK alpha 1 antibody [2B7].
Green : AMPK alpha 1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

Application Reference

Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Gao J et al. Redox Biol 2019; 28 : 101345
Application : WB
Reactivity : Mouse
Wang H et al. Free Radic Biol Med 2018; 121 : 202-214 AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409