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AMPK alpha 1 antibody [5G11]

Anti-AMPK alpha 1 antibody [5G11] used in IHC (Paraffin sections) (IHC-P). GTX34141
Anti-AMPK alpha 1 antibody [5G11] used in Western Blot (WB). GTX34141

Cat. No. GTX34141

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G11

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all AMPK alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AMPK,AMPKa1,PRKAA1,protein kinase AMPactivated catalytic subunit alpha 1,AMPK alpha 1,protein kinase AMP-activated catalytic subunit alpha 1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 antibody [5G11] used in IHC (Paraffin sections) (IHC-P). GTX34141

GTX34141 IHC-P Image

IHC-P analysis of mouse colontissue using GTX34141 AMPK alpha 1 antibody [5G11].
Dilution : 1:200

Anti-AMPK alpha 1 antibody [5G11] used in Western Blot (WB). GTX34141

GTX34141 WB Image

WB analysis of (1) HeLa, (2) 293T, (3)3T3, (4) PC12 lysates using GTX34141 AMPK alpha 1 antibody [5G11].
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399