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AMPK alpha 1 antibody [9G3]

Anti-AMPK alpha 1 antibody [9G3] used in IHC (Paraffin sections) (IHC-P). GTX34142
Anti-AMPK alpha 1 antibody [9G3] used in Western Blot (WB). GTX34142
Anti-AMPK alpha 1 antibody [9G3] used in IHC (Paraffin sections) (IHC-P). GTX34142

Cat No. GTX34142

Host Mouse
Clonality Monoclonal
Clone Name 9G3
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Specificity/Sensitivity

AMPKα1 Mouse Monoclonal Antibody (9G3) detects endogenous levels of PRKAA1
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant Protein of AMPKα1

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase Amp-Activated Catalytic Subunit Alpha 1 , Ampk , Ampka1 , Prkaa1

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-AMPK alpha 1 antibody [9G3] used in IHC (Paraffin sections) (IHC-P). GTX34142

GTX34142 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Skeletal Muscle Tissue using AMPk a1 Mouse Monoclonal Antibody diluted at 1:200.

Anti-AMPK alpha 1 antibody [9G3] used in Western Blot (WB). GTX34142

GTX34142 WB Image

Western Blot (WB) analysis of 1) HeLa Cell Lysate, 2)Mouse Brain Tissue Lysate, 3) Rat Brain Tissue Lysate using AMPK a1 Mouse Monoclonal Antibody diluted at 1:2000.

Anti-AMPK alpha 1 antibody [9G3] used in IHC (Paraffin sections) (IHC-P). GTX34142

GTX34142 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Lung Carcinoma Tissue using AMPk a1 Mouse Monoclonal Antibody diluted at 1:200.

REFERENCE
REVIEW
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Package List Price ($)
$ 319