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AMPK alpha 1 antibody

Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999
Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999

Cat. No. GTX112999

3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AMPK alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

SK-N-AS , 293T , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AMPK alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174570

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999

GTX112999 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 antibody (GTX112999) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999

GTX112999 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 antibody [N3C3] (GTX112999) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

AMPK alpha 1 antibody Cat. No. GTX112999

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999
Date : Anonymous submitted on 18-Dec-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 100μg
Blocking : 2%BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, > 16Hr
Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999
Date : Anonymous submitted on 22-Dec-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 35μg
Blocking : 5% milk in TBST, 26°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX112999
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 20 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169