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AMPK alpha 1 antibody

Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX23759

Cat. No. GTX23759

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, IHC

Reactivity

Human, Mouse, Rat, Bovine
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2μg/ml
IP Assay dependent
IHC 0.2- 2μg/ml
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Chicken, Guinea pig, Pig, Monkey, Japanese Medaka, Chimpanzee, Gorilla, Horse, Orangutan, Panda, Turkey, West Indian ocean coelacanth, Nile tilapia, Duckbill platypus, Three-spined stickleback(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH7.0-8.0

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide representing a portion of the protein encoded within exon 8.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_367630

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AMPK , AMPKa1 , PRKAA1 , protein kinase AMPactivated catalytic subunit alpha 1 , AMPK alpha 1 , protein kinase AMP-activated catalytic subunit alpha 1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 antibody used in Western Blot (WB). GTX23759

GTX23759 WB Image

WB analysis of (1) bovine aortic endothelial cell, (2) rat aortic smooth muscle cell, (3) HepG2 cell, and (4) human aortic endothelial cell lysates using GTX23759 AMPK alpha 1 antibody.
Dilution : 2μg/ml

REFERENCE

REVIEW

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Package List Price ($)
$ 399